Attenuation of mouse mesangial cell contractility by high glucose and mannitol: Involvement of protein kinase C and focal adhesion kinase
作者: Jin-Shuen ChenHerng-Sheng LeeJong-Shiaw JinAnn ChenShih-Hua LinShuk-Man KaYuh-Feng Lin
作者单位: 1Graduate Institute of Medical Sciences, National Defense Medical Center
2Graduate Institute of Life Sciences, National Defense Medical Center
3Department of Pathology, Tri-Service General Hospital
4Division of Nephrology, Department of Internal Medicine, Tri-Service General Hospital
刊名: Journal of Biomedical Science, 2004, Vol.11 (2), pp.142-151
来源数据库: Springer Journal
DOI: 10.1007/BF02256557
关键词: Mesangial cellHyperglycemiaMannitolProtein kinase CFocal adhesion kinase
英文摘要: Abstract(#br)Hyperglycemia and mannitol activate protein kinase C (PKC) and induce mesangial cell hypocontractility that subsequently may modulate renal function. Since focal adhesion kinase (FAK) activation is known to be linked with PKC activity, FAK may also be involved in mesangial cell contraction. To facilitate our understanding of the PKC- and FAK-modulating mechanism, we developed an in vitro model of mouse mesangial cell hypocontractility induced by hyperglycemia or mannitol. Mouse mesangial cells (CRL-1927) were exposed to: normal D -glucose (group N), high D -glucose (group H), and control groups at the same osmolality as H plus L -glucose (group L) and mannitol (group M). Changes in the planar surface area of cells in response to 1 µ M phorbol 12-myristate 13-acetate (PMA)...
全文获取路径: Springer  (合作)
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影响因子:2.458 (2012)

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关键词翻译
关键词翻译
  • protein 蛋白质
  • contractility 收缩性
  • kinase 激酶
  • adhesion 粘附
  • glucose 葡萄糖
  • focal 焦点的
  • mannitol 甘露糖醇
  • mouse 小鼠