Exploitation of colinear relationships between the genomes of Lotus japonicus , Pisum sativum and Arabidopsis thaliana , for positional cloning of a legume symbiosis gene
作者: S. StrackeS. SatoN. SandalM. KoyamaT. KanekoS. TabataM. Parniske
作者单位: 1The Sainsbury Laboratory, John Innes Centre
2Kazusa DNA Research Institute
3Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus
4John Innes Centre, Department of Crop Genetics
刊名: Theoretical and Applied Genetics, 2004, Vol.108 (3), pp.442-449
来源数据库: Springer Nature Journal
DOI: 10.1007/s00122-003-1438-2
英文摘要: Abstract(#br)The Lotus japonicus LjSYM2 gene, and the Pisum sativum orthologue PsSYM19 , are required for the formation of nitrogen-fixing root nodules and arbuscular mycorrhiza. Here we describe the map-based cloning procedure leading to the isolation of both genes. Marker information from a classical AFLP marker-screen in Lotus was integrated with a comparative genomics approach, utilizing Arabidopsis genome sequence information and the pea genetic map. A network of gene-based markers linked in all three species was identified, suggesting local colinearity in the region around LjSYM2/PsSYM19 . The closest AFLP marker was located just over 200 kb from the LjSYM2 gene, the marker SHMT, which was converted from a marker on the pea map, was only 7.9 kb away. The LjSYM2/PsSYM19 region...
全文获取路径: Springer Nature  (合作)
影响因子:3.658 (2012)

  • colinear 同线的
  • Lotus 美国Lotus公司
  • between 在中间
  • positional 位置
  • Arabidopsis 阿布属
  • symbiosis 共生
  • legume 豆荚
  • cloning 无性繁殖系化