Proteomic analysis of long-term salinity stress-responsive proteins in Thellungiella halophila leaves
作者: Fei GaoYiJun ZhouLingYun HuangDaCheng HeGenFa Zhang
作者单位: 1Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University
刊名: Chinese Science Bulletin, 2008, Vol.53 (22), pp.3530-3537
来源数据库: Springer Nature Journal
DOI: 10.1007/s11434-008-0455-6
关键词: Thellungiella halophilaproteomicssalinity stressmass spectrometrytwo-dimensional gel electrophoresis
英文摘要: Abstract(#br)Salinity is one of the most severe environmental factors that may impair crop productivity. A proteomic study based on two-dimensional gel electrophoresis is performed in order to analyze the long-term salinity stress response of Thellungiella halophila , an Arabidopsis -related halophyte. Four-week-old seedlings are exposed to long-term salinity treatment. The total crude proteins are extracted from leaf blades, separated by 2-DE, stained with Coomassie Brilliant Blue, and differentially displayed spots are identified by MALDI-TOF MS or QTOF MS/MS. Among 900 protein spots reproducibly detected on each gel, 30 spots exhibit significant change and some of them are identified. The identified proteins include not only some previously characterized stress-responsive proteins such...
全文获取路径: Springer Nature  (合作)
影响因子:1.319 (2012)

  • responsive 承担责任
  • salinity 盐度
  • analysis 分析
  • proteins 蛋白质
  • stress 应力