CD200 attenuates methamphetamine-induced microglial activation and dopamine depletion
作者: Xia YueDongfang QiaoAifeng WangXiaohui TanYanhong LiChao LiuHuijun Wang
作者单位: 1Department of Forensic Science, Southern Medical University
2Department of Forensic science, Nanchang University
3Guangzhou Criminal Science & Technology Institute
刊名: Journal of Huazhong University of Science and Technology [Medical Sciences], 2012, Vol.32 (3), pp.415-421
来源数据库: Springer Nature Journal
DOI: 10.1007/s11596-012-0072-0
关键词: methamphetaminemicrogliacluster of differentiation molecule 200dopaminestriatum
英文摘要: Summary(#br)This study examined the neuroprotective effect of cluster of differentiation molecule 200 (CD200) against methamphetamine (METH)-induced neurotoxicity. In the in vitro experiment, neuron-microglia cultures were treated with METH (20 μmol/L), METH (20 μmol/L)+CD200-Fc (10 μg/mL) or CD200-Fc (10 μg/mL). Those untreated served as control. Microglia activation expressed as the ratio of MHC-II/CD11b was assessed by flow cytometry. The cytokines (IL-1β, TNF-α) secreted by activated microglia were detected by enzyme-linked immunosorbent assay (ELISA). In the in vivo experiment, 40 SD rats were divided into control, METH, METH+CD200-Fc and CD200-Fc groups at random. Rats were intraperitoneally injected with METH (15 mg/kg 8 times at 12 h interval) in METH group, with METH...
全文获取路径: Springer Nature  (合作)

  • dopamine 多巴胺
  • induced 感应的
  • activation 活化
  • depletion 矿量递减