Exploring the Transcriptome of Atlantic Salmon ( Salmo salar ) Skin, a Major Defense Organ
作者: Giulia MicallefRalph BickerdikeCaroline ReiffJorge M. O. FernandesAlan S. BowmanSamuel A. M. Martin
作者单位: 1Institute of Biological and Environmental Sciences, University of Aberdeen
2BioMar Ltd
3School of Medicine & Dentistry, Division of Applied Medicine, University of Aberdeen
4Faculty of Biosciences and Aquaculture, University of Nordland
刊名: Marine Biotechnology, 2012, Vol.14 (5), pp.559-569
来源数据库: Springer Journal
DOI: 10.1007/s10126-012-9447-2
关键词: 454-SequencingMucinAquacultureSkin
英文摘要: Abstract(#br)The skin of fish is the first line of defense against pathogens and parasites. The skin transcriptome of the Atlantic salmon is poorly characterized, and currently only 2,089 expressed sequence tags (ESTs) out of a total of half a million sequences are generated from skin-derived cDNA libraries. The primary aim of this study was to enhance the transcriptomic knowledge of salmon skin by using next-generation sequencing (NGS) technology, namely the Roche-454 platform. An equimolar mixture of high-quality RNA from skin and epidermal samples of salmon reared in either freshwater or seawater was used for 454-sequencing. This technique yielded over 600,000 reads, which were assembled into 34,696 isotigs using Newbler. Of these isotigs, 12 % had not been sequenced in Atlantic...
全文获取路径: Springer  (合作)
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影响因子:2.739 (2012)

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关键词翻译
关键词翻译
  • salar 干盐湖
  • Atlantic 在西洋的
  • Skin 皮肤