Simultaneous quantification of Pacific ciguatoxins in fish blood using liquid chromatography–tandem mass spectrometry
作者: Yim Ling MakJia Jun WuWing Hei ChanMargaret B. MurphyJames C. W. LamLeo L. ChanPaul K. S. Lam
作者单位: 1State Key Laboratory in Marine Pollution, City University of Hong Kong
2Department of Biology and Chemistry, City University of Hong Kong
3Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute
刊名: Analytical and Bioanalytical Chemistry, 2013, Vol.405 (10), pp.3331-3340
来源数据库: Springer Journal
DOI: 10.1007/s00216-013-6766-8
关键词: Ciguatera fish poisoningPacific ciguatoxinsP-CTXsFish bloodLC-MS/MS
英文摘要: Abstract(#br)Ciguatera fish poisoning (CFP) is a food intoxication caused by exposure to ciguatoxins (CTXs) in coral reef fish. Rapid analytical methods have been developed recently to quantify Pacific-CTX-1 (P-CTX-1) in fish muscle, but it is destructive and can cause harm to valuable live coral reef fish. Also fish muscle extract was complex making CTX quantification challenging. Not only P-CTX-1, but also P-CTX-2 and P-CTX-3 could be present in fish, contributing to ciguatoxicity. Therefore, an analytical method for simultaneous quantification of P-CTX-1, P-CTX-2, and P-CTX-3 in whole blood of marketed coral reef fish using sonication, solid-phase extraction (SPE), and liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. The optimized method gave acceptable...
全文获取路径: Springer  (合作)
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来源刊物:
影响因子:3.659 (2012)

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关键词翻译
关键词翻译
  • liquid 液体
  • chromatography 色层分离法
  • tandem 串联机
  • spectrometry 光谱测定
  • Pacific 太平洋
  • quantification 量化
  • blood 血液