1-(3-Aminopropyl)-3-butylimidazolium bromide for carboxyl group derivatization: potential applications in high sensitivity peptide identification by mass spectrometry
作者: XiaoQiang QiaoYuan ZhouChunYan HouXiaoDan ZhangKaiGuang YangLiHua ZhangYuKui Zhang
作者单位: 1Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
2Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmaceutical Sciences, Hebei University
刊名: Science China Life Sciences, 2013, Vol.56 (3), pp.240-245
来源数据库: Springer Nature Journal
DOI: 10.1007/s11427-013-4446-8
关键词: 1-(3-aminopropyl)-3-butylimidazolium bromideionization capacitypeptidemass spectrometry analysisderivatization
英文摘要: Abstract(#br)The cationic reagent 1-(3-aminopropyl)-3-butylimidazolium bromide (BAPI) was exploited for the derivatization of carboxyl groups on peptides. Nearly 100% derivatization efficiency was achieved with the synthetic peptide RVYVHPI (RI-7). Furthermore, the peptide derivative was stable in a 0.1% TFA/water solution or a 0.1% (v/v) TFA/acetonitrile/water solution for at least one week. The effect of BAPI derivatization on the ionization of the peptide RI-7 was further investigated, and the detection sensitivity was improved >42-fold via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), thus outperforming the commercial piperazine derivatization approach. Moreover, the charge states of the peptide were largely increased via BAPI...
全文获取路径: Springer Nature  (合作)
影响因子:2.024 (2011)

  • carboxyl 羧基
  • derivatization 衍生作用
  • peptide 
  • potential 
  • sensitivity 灵敏度
  • spectrometry 光谱测定
  • identification 辨认
  • bromide 溴化物
  • group 
  • applications 应用程序