Identification and Characterization of an Extracellular Alkaline Phosphatase in the Marine Diatom Phaeodactylum tricornutum
作者: Hung-Yun LinChi-Yu ShihHung-Chun LiuJeng ChangYing-Lan ChenYet-Ran ChenHan-Tso LinYu-Yung ChangChun-Hua HsuHan-Jia Lin
作者单位: 1Institute of Bioscience and Biotechnology, National Taiwan Ocean University
2Institute of Marine Biology, National Taiwan Ocean University
3Center of Excellence for Marine Bioenvironment and Biotechnology, National Taiwan Ocean University
4Department of Life Science and Institute of Plant Biology, National Taiwan University
5Agricultural Biotechnology Research Center, Academia Sinica
6Department of Biotechnology, Ming-Chuan University
7Department of Agricultural Chemistry, National Taiwan University
刊名: Marine Biotechnology, 2013, Vol.15 (4), pp.425-436
来源数据库: Springer Journal
DOI: 10.1007/s10126-013-9494-3
关键词: Alkaline phosphataseDiatomGlycoproteinMetal ionPhoA
英文摘要: Abstract(#br)In phosphorus-deficient conditions, Phaeodactylum tricornutum releases an alkaline phosphatase (PtAPase) to the medium that is readily detectable by activity staining. Nucleic acid and amino acid sequence of this alkaline phosphatase (APase) was identified by performing proteomic analysis and database searches. Sequence alignment suggests that PtAPase belongs to the PhoA family, and it possesses key residues at the Escherichia coli PhoA active site. Quantitative PCR results indicate that the induction of APase mRNA transcription is very sensitive to phosphorus availability and population growth. The molecular mass of native PtAPase (148 kDa) determined by gel filtration chromatography indicates that PtAPase, like most PhoA, is homodimeric. Zn and Mg ions are essential...
全文获取路径: Springer  (合作)
影响因子:2.739 (2012)

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