Enhanced aldehyde dehydrogenase activity by regenerating NAD + in Klebsiella pneumoniae and implications for the glycerol dissimilation pathways
作者: Ying LiMingyue SuXizhen GePingfang Tian
作者单位: 1College of Life Science and Technology, Beijing University of Chemical Technology
2College of Biochemical Engineering, Beijing Union University
刊名: Biotechnology Letters, 2013, Vol.35 (10), pp.1609-1615
来源数据库: Springer Journal
DOI: 10.1007/s10529-013-1243-1
关键词: Aldehyde dehydrogenase3-Hydroxypropionic acidKlebsiella pneumoniaeNAD + regeneration
英文摘要: Abstract(#br)In Klebsiella pneumoniae , 3-hydroxypropaldehyde is converted to 3-hydroxypropionic acid (3-HP) by aldehyde dehydrogenase (ALDH) with NAD + as a cofactor. Although ALDH overexpression stimulates the formation of 3-HP, it ceases to accumulate when NAD + is exhausted. Here we show that NAD + regeneration, together with ALDH overexpression, facilitates 3-HP production and benefits cell growth. Three distinct NAD + -regenerating enzymes: NADH oxidase and NADH dehydrogenase from K . pneumoniae , and glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae , were individually expressed in K. pneumoniae . In vitro assay showed their higher activities than that of the control, indicating their capacities to regenerate NAD + . When they were respectively co-expressed...
全文获取路径: Springer  (合作)
影响因子:1.853 (2012)

  • dissimilation 分异化酌
  • dehydrogenase 脱氢酶
  • aldehyde 
  • glycerol 甘油
  • activity 活度
  • NAD Network Access Devices
  • regenerating 再生