Rapid and accurate generation of various concentration gradients using polydimethylsiloxane-sealed hydrogel device
作者: Minseok KimMingjie JiaYoungmi KimTaesung Kim
作者单位: 1School of Mechanical and Advanced Materials Engineering, Ulsan National Institute of Science and Technology (UNIST)
2School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST)
刊名: Microfluidics and Nanofluidics, 2014, Vol.16 (4), pp.645-654
来源数据库: Springer Nature Journal
DOI: 10.1007/s10404-013-1265-y
关键词: MicrofluidicsHydrogelPolydimethylsiloxaneConcentration gradientChemotaxis
英文摘要: Abstract(#br)We report a microfluidic device that can rapidly and accurately generate various concentration gradients in a controllable manner for the chemotaxis study of motile bacterial cells by integrating hydrogel into polydimethylsiloxane (PDMS) microchannels. We performed numerical simulations for both the PDMS-sealed hydrogel hybrid device and a representative conventional hydrogel-based device to theoretically compare their characteristics. In addition, we experimentally demonstrated that the PDMS-sealed hydrogel device not only produces various linear and nonlinear concentration gradients without flow-induced shear stresses on motile bacterial cells but also exhibits remarkable advantages over conventional hydrogel-based devices. For example, the PDMS-sealed hydrogel device can...
全文获取路径: Springer Nature  (合作)
影响因子:3.218 (2012)

  • sealed 封闭的
  • hydrogel 水凝胶
  • concentration 浓度
  • generation 世代
  • accurate 精确的
  • Rapid 拉皮德牌照相机
  • device 装置
  • various 千变万化的