Thermal inactivation of xanthine oxidase from Arthrobacter M3: mechanism and the corresponding thermostabilization strategy
作者: Yuran ZhangYu XinHailin YangLing ZhangXiaole XiaYanjun TongYi ChenWu Wang
作者单位: 1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University
刊名: Bioprocess and Biosystems Engineering, 2014, Vol.37 (4), pp.719-725
来源数据库: Springer Journal
DOI: 10.1007/s00449-013-1042-z
关键词: Xanthine oxidaseThermal inactivationKineticsBetaineTrehalose
英文摘要: Abstract(#br)The mechanism of thermal inactivation about xanthine oxidase (XOD) from Arthrobacter M3 was investigated. Results of reducing SDS-PAGE indicated that the inactivation of XOD was not related to the peptide degradation. Meanwhile, fluorimetry and circular dichroism spectroscopy suggested that XOD inactivation might be associated with the exposure of hydrophobic residues to surface and partial loss of secondary structure. Specific formation of soluble aggregates of XOD was detected by size exclusion chromatography. In addition, the thermal-dynamic analysis showed that the inactivation kinetics of XOD followed the first-order model. Therefore, trehalose (cosolute) and betaine (osmolyte) were accordingly employed to attenuate the inactivation of this enzyme. The results associated...
全文获取路径: Springer  (合作)
影响因子:1.869 (2012)

  • mechanism 机构
  • xanthine 黄质
  • corresponding 对应的
  • strategy 战略
  • oxidase 氧化酶
  • inactivation 失活