Distinct Promoters Affect Pyrroloquinoline Quinone Production in Recombinant Escherichia coli and Klebsiella pneumoniae
作者: Jiguo SunZengye HanXizhen GePingfang Tian
作者单位: 1Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology
2College of Biochemical Engineering, Beijing Union University
刊名: Current Microbiology, 2014, Vol.69 (4), pp.451-456
来源数据库: Springer Journal
DOI: 10.1007/s00284-014-0607-7
英文摘要: Abstract(#br)Pyrroloquinoline quinone (PQQ) is a versatile quinone cofactor participating in numerous biological processes. Klebsiella pneumoniae can naturally synthesize PQQ for harboring intact PQQ synthesis genes. Previous metabolic engineering of K . pneumoniae failed to overproduce PQQ due to the employment of strong promoter in expression vector. Here we report that a moderate rather than strong promoter is efficient for PQQ production. To screen an appropriate promoter, a total of four distinct promoters— lac promoter, pk promoter of glycerol dehydratase gene ( dhaB1 ), promoter of kanamycin resistance gene, and T7 promoter (as the control)—were individually used for overexpressing the endogenous PQQ genes in K. pneumoniae along with heterologous expression in Escherichia coli . We...
全文获取路径: Springer  (合作)
影响因子:1.52 (2012)

  • Escherichia 埃希氏菌属