NAD + -independent aldehyde oxidase catalyzes cofactor balanced 3-hydroxypropionic acid production in Klebsiella pneumoniae
作者: Ying LiLuo LiuPingfang Tian
作者单位: 1Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology
刊名: Biotechnology Letters, 2014, Vol.36 (11), pp.2215-2221
来源数据库: Springer Nature Journal
DOI: 10.1007/s10529-014-1590-6
关键词: Aldehyde dehydrogenaseAldehyde oxidaseGlycerol3-Hydroxypropionic acidKlebsiella pneumoniaeNAD +
英文摘要: Abstract(#br)The limiting step for biosynthesis of 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae is the conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-HP. This reaction is catalyzed by aldehyde dehydrogenase (ALDH) with NAD + as a cofactor. Although NAD + -dependent ALDH overexpression facilitates 3-HP biosynthesis, ALDH activity decreases and 3-HP stops accumulation when NAD + is exhausted. Here, we show that an NAD + -independent aldehyde oxidase (AOX) from Pseudomonas sp. AIU 362 holds promise for cofactor-balanced 3-HP production in K. pneumoniae . The AOX coding gene, alod , was heterologously expressed in E. coli and K. pneumoniae , and their respective crude cell extracts showed 38.1 U/mg and 16.6 U/mg activities toward propionaldehyde. The recombinant K. pneumoniae...
全文获取路径: Springer Nature  (合作)
影响因子:1.853 (2012)

  • cofactor 辅助因子
  • NAD Network Access Devices
  • aldehyde 
  • independent 独立的
  • production 生产
  • balanced 平[均]衡
  • oxidase 氧化酶