Agar coating in multicellular spheroids culture of rat hepatocytes
作者: Shaolin LiJilun PanTao XuYaoting Yu
作者单位: 1Bioactive Materials Research Laboratory, Institute for Molecular Biology, Nankai University
刊名: Chinese Science Bulletin, 1999, Vol.44 (24), pp.2246-2249
来源数据库: Springer Journal
DOI: 10.1007/BF02885931
关键词: agarhepatocytemulticellular spheroids
英文摘要: Abstract(#br)Aggregation of freshly isolated adult rat hepatocytes in vitro is important for the construction of artificial liver support system. In the experiment, agar has been used as an extracellular matrix substrate and results demonstrate that hepatocytes in serum-free culture medium can effectively form floating spheroids in tissue culture dishes coated with agar, which maintain higher ability to produce urea and albumin than monolayer cultured cells.
全文获取路径: Springer  (合作)
影响因子:1.319 (2012)

  • multicellular 多细胞的
  • coating 覆盖层
  • culture