Transgenic studies reveal the positive role of LeEIL-1 in regulating shikonin biosynthesis in Lithospermum erythrorhizon hairy roots
作者: Rongjun FangAilan ZouHua ZhaoFengyao WuYu ZhuHu ZhaoYonghui LiaoRen-Jie TangYanjun PangRongwu YangXiaoming WangJinliang QiGuihua LuYonghua Yang
作者单位: 1Nanjing University
2Jiangsu University of Science and Technology
3Nanjing Forestry University
4University of California
刊名: BMC Plant Biology, 2016, Vol.16 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12870-016-0812-6
关键词: EthyleneHairy rootsLeEIL-1OverexpressionRNAiShikonin
英文摘要: Abstract(#br) Background(#br)The phytohormone ethylene (ET) is a key signaling molecule for inducing the biosynthesis of shikonin and its derivatives, which are secondary metabolites in Lithospermum erythrorhizon . Although ETHYLENE INSENSITIVE3 (EIN3)/EIN3-like proteins (EILs) are crucial transcription factors in ET signal transduction pathway, the possible function of EIN3/EIL1 in shikonin biosynthesis remains unknown. In this study, by targeting LeEIL-1 ( L. erythrorhizon EIN3-like protein gene 1) at the expression level, we revealed the positive regulatory effect of LeEIL-1 on shikonin formation.(#br) Results(#br)The mRNA level of LeEIL-1 was significantly up-regulated and down-regulated in the LeEIL-1 -overexpressing hairy root lines and LeEIL-1 -RNAi hairy root lines, respectively....
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影响因子:4.354 (2012)

  • regulating 蝶的
  • roots 麻根
  • biosynthesis 生物合成
  • reveal 显露
  • hairy 多毛的
  • positive 阳片