Enhanced Promoter Activity by Replenishment of Sigma Factor rpoE in Klebsiella pneumoniae
作者: Liuni ChenYing LiPingfang Tian
作者单位: 1Beijing University of Chemical Technology
刊名: Indian Journal of Microbiology, 2016, Vol.56 (2), pp.190-197
来源数据库: Springer Journal
DOI: 10.1007/s12088-016-0576-6
关键词: Klebsiella pneumoniaerpoE3-Hydroxypropionic acidAldehyde dehydrogenaseGlycerol
英文摘要: Abstract(#br)Plasmid-dependent overexpression of enzyme(s) aims to divert carbon flux toward a desired compound. One drawback of this strategy is compromise of growth due to massive consumption of host resources. Here we show that replenishment of sigma factor rpoE improves the growth of Klebsiella pneumoniae . The gene rpoE was expressed alone or coexpressed with Ald4 (an aldehyde dehydrogenase from Saccharomyces cerevisiae ) in K . pneumoniae . We found that the Ald4 activity was higher in the strain coexpressing Ald4 and rpoE (32.3 U/mg) than that expressing Ald4 alone (29.9 U/mg). Additionally, under shake-flask conditions, the strain coexpressing Ald4 and rpoE produced 0.5 g 3-hydroxypropionic acid (3-HP) and 9.8 g 1,3-propanediol (1,3-PD) per liter in 24 h, which were 1.6- and...
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影响因子:0.457 (2012)

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