3-Hydroxypropionaldehyde-specific aldehyde dehydrogenase from Bacillus subtilis catalyzes 3-hydroxypropionic acid production in Klebsiella pneumoniae
作者: Mingyue SuYing LiXizhen GePingfang Tian
作者单位: 1Beijing University of Chemical Technology
2Beijing Union University
刊名: Biotechnology Letters, 2015, Vol.37 (3), pp.717-724
来源数据库: Springer Journal
DOI: 10.1007/s10529-014-1730-z
关键词: Aldehyde dehydrogenaseGlycerol3-Hydroxypropionaldehyde3-Hydroxypropionic acidKlebsiella pneumoniaeSubstrate specificity
英文摘要: Abstract(#br)In Klebsiella pneumoniae , aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae , the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we report that DhaS, a putative ALDH from Bacillus subtilis , shows high specificity toward 3-HPA when heterologously expressed in K . pneumoniae . Using NAD+ as a cofactor, DhaS exhibited higher catalytic activity (2.3 U mg−1) and lower K m value (0.4 mmol l−1) toward 3-HPA than that toward other aldehydes. Under shake-flask conditions, the recombinant strain produced 2.1 g 3-HP l−1 in 24 h, which is 3.9-fold of...
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影响因子:1.853 (2012)

  • aldehyde 
  • dehydrogenase 脱氢酶
  • specific 
  • production 生产
  • subtilis 微小