Enhancement of 3-hydroxypropionic acid production from glycerol by using a metabolic toggle switch
作者: Keigo TsurunoHiroshi HonjoTaizo Hanai
作者单位: 1Kyushu University
刊名: Microbial Cell Factories, 2015, Vol.14 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12934-015-0342-1
关键词: 3-Hydroxypropionic acidEscherichia coliMetabolic toggle switchSynthetic pathwayGlycerolSynthetic genetic circuit
英文摘要: Abstract(#br) Background(#br)3-hydroxypropionic acid (3-HP) is an important platform for the production of C3 chemicals, including acrylic acid, methyl acrylate, and acrylamide. Microbial production of 3-HP is mainly due to glycerol metabolism. In this study, in order to improve microbial 3-HP production, we applied a metabolic toggle switch for controlling the glycerol metabolism to redirect the excess metabolic flux of central metabolic pathway toward an exogenous 3-HP producing pathway in Escherichia coli .(#br) Results(#br)The metabolic toggle switch enables conditional repression of the expression of a target gene during the fermentation. We individually performed conditional repression of glpK , tpiA , and gapA, which are involved in glycerol metabolism. The conditional repression...
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影响因子:3.306 (2012)

  • toggle 解发器反复电路
  • production 生产
  • switch 开关
  • glycerol 甘油
  • metabolic 新陈代谢的