Efficient whole-cell catalysis for 5-aminovalerate production from L-lysine by using engineered Escherichia coli with ethanol pretreatment
乙醇预处理工程大肠杆菌高效全细胞催化赖氨酸生产5-氨基戊酸
作者: Jie ChengQing LuoHuaichuan DuanHao PengYin ZhangJianping HuYao Lu
作者单位: 1Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, College of Pharmacy and Biological Engineering, Chengdu University, 610106, Chengdu, P. R. China
2College of Chemistry and Chemical Engineering, Yangtze Normal University, 408100, Chongqing, P. R. China
3College of Biological and Chemical Engineering, Guangxi University of Science and Technology, 545006, Liuzhou, P. R. China
刊名: Scientific Reports, 2020, Vol.10 (1), pp.19-890
来源数据库: Springer Nature Journal
DOI: 10.1038/s41598-020-57752-x
英文摘要: Abstract(#br)Microorganisms can utilize biomass to produce valuable chemicals, showing sustainable, renewable and economic advantages compared with traditional chemical synthesis. As a potential five-carbon platform polymer monomer, 5-aminovalerate has been widely used in industrial fields such as clothes and disposable goods. Here we establish an efficient whole-cell catalysis for 5-aminovalerate production with ethanol pretreatment. In this study, the metabolic pathway from L-lysine to 5-aminovalerate was constructed at the cellular level by introducing L-lysine α-oxidase. The newly produced H2O2 and added ethanol both are toxic to the cells, obviously inhibiting their growth. Here, a promising strategy of whole-cell catalysis with ethanol pretreatment is proposed,...
全文获取路径: Springer Nature  (合作)
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影响因子:2.927 (2012)

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