Analysis of viral diversity in stool samples from infants and children with acute gastroenteritis in Kuwait using Metagenomics approach
科威特婴幼儿急性胃肠炎粪便中病毒多样性的亚基因组分析
作者: Hawraa Adel MohammadNada Mohammed MadiWidad Al-Nakib
作者单位: 1Virology Unit, Department of Microbiology, Faculty of Medicine, Kuwait University, P.O.Box 24923, 13110, Safat, Kuwait
刊名: Virology Journal, 2020, Vol.17 (12), pp.O182-O187
来源数据库: Springer Nature Journal
DOI: 10.1186/s12985-020-1287-5
关键词: Next-generation sequencingMetagenomics approachViral gastroenteritisKuwait
英文摘要: Abstract(#br)Background(#br)Current molecular target-dependent methods are used to detect only known viruses. However, metagenomics based on next-generation sequencing (NGS) technique is a target-independent assay that enables simultaneous detection and genomic characterisation of all microorganisms present in a sample. In this study, we aimed to develop a metagenomics approach using NGS to identify and characterise viruses in stool samples from infants and children with Acute Gastroenteritis (AGE) in Kuwait. Methods(#br)We have investigated 84 stool samples from infants and children aged one month to ten years old with signs and symptoms of gastroenteritis who attended Mubarak Al-Kabeer and Al-Amiri hospitals in Kuwait from January to December 2017. A metagenomics approach using NGS to...
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影响因子:2.092 (2012)

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