NFкB is a critical transcriptional regulator of atypical cadherin FAT1 in glioma
作者: Chitrangda SrivastavaKhushboo IrshadYakhlesh GuptaChitra SarkarAshish SuriParthaprasad ChattopadhyaySubrata SinhaKunzang Chosdol
作者单位: 1Department of Biochemistry, All India Institute of Medical Sciences, -110029, New Delhi, India
2Present address: Cell Biology, National Institute of Environmental Health Sciences, National Institutes of Health, 27709, Durham, NC, USA
3Department of Pathology, All India Institute of Medical Sciences, New Delhi, India
4Department of Neurosurgery, All India Institute of Medical Sciences, New Delhi, India
刊名: BMC Cancer, 2020, Vol.20 (6 Pt 1), pp.733-736
来源数据库: Springer Nature Journal
DOI: 10.1186/s12885-019-6435-1
关键词: FAT1NFкB (RelA)GlioblastomaTumorPromoter
英文摘要: Abstract(#br)Background(#br)Overexpression of FAT1 gene and its oncogenic effects have been reported in several cancers. Previously, we have documented upregulation of FAT1 gene in glioblastoma (GBM) tumors which was found to increase the expression of proinflammatory markers, HIF-1α, stemness genes and EMT markers in glioma cells. Here, we reveal NFкB (RelA)/RelA/p65 as the transcriptional regulator of FAT1 gene in GBM cells. Methods(#br)In-silico analysis of FAT1 gene promoter was performed using online bioinformatics tool Promo alggen (Transfac 8.3) to identify putative transcription factor(s) binding motifs. A 4.0 kb FAT1 promoter (− 3220 bp to + 848 bp w.r.t. TSS + 1) was cloned into promoter less pGL3Basic reporter vector. Characterization of FAT1 promoter for transcriptional...
全文获取路径: Springer Nature  (合作)
影响因子:3.333 (2012)