Bacillus velezensis LG37: transcriptome profiling and functional verification of GlnK and MnrA in ammonia assimilation
作者: Guangxin LiuSarath Babu VijayaramanYanjun DongXinfeng LiBinda Tembeng AndongmaaLijuan ZhaoJiagang TuJin HeLi Lin
作者单位: 1State Key Laboratory of Agricultural Microbiology, College of Fisheries and College of Life Sciences, Huazhong Agricultural University, 430070, Wuhan, Hubei Province, China
2Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, 510225, Guangzhou, Guangdong, China
3Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Marine Science and Technology, 266071, Qingdao, Shandong, China
刊名: BMC Genomics, 2020, Vol.21 (Database), pp.115-122
来源数据库: Springer Nature Journal
DOI: 10.1186/s12864-020-6621-1
关键词: Bacillus velezensisTranscriptomeTransporterCRISPR-Cas9Ammonium nitrogenAmmonia assimilation pathway
英文摘要: Abstract(#br)Background(#br)In recent years, interest in Bacillus velezensis has increased significantly due to its role in many industrial water bioremediation processes. In this study, we isolated and assessed the transcriptome of Bacillus velezensis LG37 (from an aquaculture pond) under different nitrogen sources. Since Bacillus species exhibit heterogeneity, it is worth investigating the molecular mechanism of LG37 through ammonia nitrogen assimilation, where nitrogen in the form of molecular ammonia is considered toxic to aquatic organisms. Results(#br)Here, a total of 812 differentially expressed genes (DEGs) from the transcriptomic sequencing of LG37 grown in minimal medium supplemented with ammonia (treatment) or glutamine (control) were obtained, from which 56 had Fold Change ≥2....
全文获取路径: Springer Nature  (合作)
影响因子:4.397 (2012)