XIST promotes cell proliferation and invasion by regulating miR-140-5p and SOX4 in retinoblastoma
作者: Yuhui WangDahong SunYing ShengHong GuoFanchun MengTingting Song
作者单位: 1Department of Clinical Laboratory, Affiliated Hospital of Beihua University, 132011, Jilin, China
2Department of Pediatrics, The Third People’s Hospital of Qingdao, 266041, Qingdao, China
3Health Management Center, The People’s Hospital of Zhangqiu Area, 250200, Jinan, China
4Delivery Room, The People’s Hospital of Zhangqiu Area, 250200, Jinan, China
5No. 2 Department of Oncology, Qingdao Central Hospital, Qingdao Tumor Hospital, 127 Siliu South Road, Shibei District, 266042, Qingdao, China
刊名: World Journal of Surgical Oncology, 2020, Vol.18 (1), pp.201-223
来源数据库: Springer Nature Journal
DOI: 10.1186/s12957-020-01825-8
关键词: RetinoblastomaXISTMiR-140-5pSOX4
原始语种摘要: Abstract(#br)Background(#br)Retinoblastoma (RB) is the most common intraocular malignancy in children. Long non-coding RNA X-inactive specific transcript (lncRNA XIST) has been reported to be associated with RB, but research on the mechanism of XIST is not well studied. Methods(#br)Expressions of XIST, microRNA-140-5p (miR-140-5p), and sex-determining region Y-related high-mobility group box 4 (SOX4) were analyzed by qRT-PCR or Western blot. Relationships of XIST, SOX4, and miR-140-5p were evaluated by dual-luciferase reporter assay and Spearman’s analysis. Cell Counting Kit-8 (CCK-8) and Transwell assay were performed to assess the function of XIST on RB cell proliferation and invasion. Results(#br)In RB tissues, XIST and SOX4 expressions were obviously increased, but the miR-140-5p...
全文获取路径: Springer Nature  (合作)
影响因子:1.092 (2012)