miR-765 inhibits the osteogenic differentiation of human bone marrow mesenchymal stem cells by targeting BMP6 via regulating the BMP6/Smad1/5/9 signaling pathway
作者: Tao WangChao ZhangCihu WuJianyun LiuHui YuXiaoou ZhouJie ZhangXinping WangShan HeXiaoyuan XuBaicheng MaXiangxin CheWeidong Li
作者单位: 1Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, 332000, Jiujiang, China
2Breast surgery, Affiliated Hospital of Jiujiang University, 332000, Jiujiang, China
3Medical department, Jiujiang University, 332000, Jiujiang, China
刊名: Stem Cell Research & Therapy, 2020, Vol.11 (11), pp.143-147
来源数据库: Springer Nature Journal
DOI: 10.1186/s13287-020-1579-0
关键词: MiR-765Human bone marrow mesenchymal stem cellsOsteogenic differentiationBone morphogenetic protein 6
英文摘要: Abstract(#br)Background(#br)The process of bone repair is heavily dependent on the ability of human bone marrow mesenchymal stem cells (hMSCs) to undergo osteogenic differentiation. MicroRNAs have been shown to regulate this osteogenic process. This study aimed to investigate the role of miR-765 in the osteogenic differentiation of hMSCs. Methods(#br)We transfected hMSCs with lentiviral constructs to knock down or overexpress this miRNA, allowing us to assess its role in osteogenesis via assessing the expression of the relevant markers alkaline phosphatase (ALP), runt-related gene-2 (RUNX-2), and osteocalcin (OCN), with further functional measurements made via quantifying ALP activity and conducting Alizarin Red S staining. The targeting of the 3′-untranslated region (UTR) of BMP6 by...
全文获取路径: Springer Nature  (合作)
影响因子:3.652 (2012)