Two novel protein chips for the detection of antibodies against porcine parvovirus
作者: Yue WuXudan WuJinxiu HouXiongnan ChenXiaobo HuangBin Zhou
作者单位: 1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, 210095, Nanjing, China
2College of Veterinary Medicine, Yangzhou University, 225009, Yangzhou, China
3College of Veterinary Medicine, Sichuan Agricultural University, 611130, Chengdu, China
刊名: BMC Veterinary Research, 2020, Vol.16 (6), pp.371-377
来源数据库: Springer Nature Journal
DOI: 10.1186/s12917-020-02280-z
关键词: Protein chipPorcine parvovirus (PPV)Antibody detectionClinical serum
英文摘要: Abstract(#br)Background(#br)PPV is one of the most important pathogens causing porcine reproductive disorder. It has been shown in clinical cases to be a commonly mixed infection with other important swine diseases which can aggravate the severity of the disease and bring serious economic losses to the pig industry. Serological methods, such as hemagglutination inhibition assays (HAI), serum neutralization (SN), and the modified direct complement-fixation (MDCF) test were utilized earlier, whereas the enzyme-linked immunosorbent assay (ELISA) is the most frequently applied assay to detect PPV-specific antibodies. Results(#br)We establish the visible protein chip and the cyanine dye 3 (Cy3)-labeled protein chip to detect the clinical serum from pigs. In this study, the recombinant protein...
全文获取路径: Springer Nature  (合作)
影响因子:1.861 (2012)