Two-step derivatization for determination of sugar phosphates in plants by combined reversed phase chromatography/tandem mass spectrometry
作者: Umut RendeTotte NiittyläThomas Moritz
刊名: Plant Methods, 2019, Vol.15 (1), pp.1
来源数据库: Springer Nature Journal
DOI: 10.1186/s13007-019-0514-9
关键词: Sugar phosphatesUHPLC–ESI–MSQ-TOFQqQ-MSExtractionTwo-step derivatization
原始语种摘要: Sugar phosphates are important intermediates of central carbon metabolism in biological systems, with roles in glycolysis, the pentose–phosphate pathway, tricarboxylic acid (TCA) cycle, and many other biosynthesis pathways. Understanding central carbon metabolism requires a simple, robust and comprehensive analytical method. However, sugar phosphates are notoriously difficult to analyze by traditional reversed phase liquid chromatography. Here, we show a two-step derivatization of sugar phosphates by methoxylamine and propionic acid anhydride after chloroform/methanol (3:7) extraction from Populus leaf and developing wood that improves separation, identification and quantification of sugar phosphates by ultra high performance liquid chromatography–electrospray ionization–mass spectrometry...
全文获取路径: Springer Nature  (合作)
影响因子:2.667 (2012)

  • derivatization 衍生作用
  • chromatography 色层分离法
  • reversed 倒转的
  • phase 相位
  • sugar 
  • liquid 液体
  • spectrometry 光谱测定
  • tandem 串联机
  • anhydride 无水物
  • combined 组合