Dual amplification in a fluorometric acetamiprid assay by using an aptamer, G-quadruplex/hemin DNAzyme, and graphene quantum dots functionalized with D-penicillamine and histidine
作者: Li NanaLi RuiyiSun XiulanYang YongqiangLi Zaijun
刊名: Microchimica Acta, 2020, Vol.187 (3), pp.158
来源数据库: Springer Nature Journal
DOI: 10.1007/s00604-020-4127-9
关键词: NanoprobeEnzyme free amplificationRatiometric measureNeonicotinoid insecticides
原始语种摘要: D-penicillamine and histidine-functionalized graphene quantum dot (DPA-GQD-His was synthesized and applied in a fluorometric method for determination of acetamiprid using a G-quadruplex DNAzyme. At first DNA probe (probe 1 consists of a target-specific aptamer with two arms of DNA segments. Probe 1 was hybridized with DNA probe 2 composed of a single DNA sequence with two split G-rich DNA sequences. This leads to the formation of a triplex-to-G-quadruplex (TPGQ. Next, acetamiprid was hybridized with the aptamer in the TPGQ to release free DNA probe 2. The released probe 2, in the presence of of K, undergoes a structural change into a stem-loop structure (by self-complementary hybridization and Hoogsteen hydrogen bonding that bears a G-quadruplex structure. This is followed by conjugation...
全文获取路径: Springer Nature  (合作)
影响因子:3.434 (2012)