Successfully transfected primary peripherally mobilized human CD34+ hematopoietic stem and progenitor cells (HSPCs) demonstrate increased susceptibility to retroviral infection
作者: Jeffrey SebrowStephen P. GoffDaniel O. Griffin
作者单位: 1Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, 701 West 168th Street, HHSC 1310, 10032, New York, NY, USA
2HHMI, Department of Biochemistry and Molecular Biophysics, and Department of Microbiology and Immunology, Columbia University Medical Center, 10032, New York, NY, USA
3Department of Medicine, Division of Infectious Diseases, Columbia University, College of Physicians and Surgeons, 10032, New York, NY, USA
刊名: Virology Journal, 2020, Vol.17 (820–832), pp.456-467
来源数据库: Springer Nature Journal
DOI: 10.1186/s12985-020-1297-3
关键词: TransfectionTransductionCD34 +Stem cellsProgenitor cells
原始语种摘要: Abstract(#br)Transfection, the process of introducing purified nucleic acids into cells, and viral transduction, viral-mediated nucleic acid transfer, are two commonly utilized techniques for gene delivery in the research setting. Transfection allows purified nucleic acid to be introduced into target cells through chemical-based techniques, nonchemical methods or particle-based methods, while viral transduction employs genomes or vectors based on adenoviruses, retroviruses (e.g. lentiviruses), adeno-associated viruses, or hybrid viruses. Transfected DNAs are often tested for potential effects on subsequent transduction, but it is not clear whether transfection itself rather than the particular nucleic acid being introduced might impact subsequent viral transfection. We observed a...
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影响因子:2.092 (2012)

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