Automated quantification of Epstein-Barr virus in whole blood for post-transplant lymphoproliferative disorders monitoring
全血Epstein-Barr病毒自动定量检测在移植后淋巴增生性疾病监测中的应用
作者: Maud SalmonaKarl SteficNadia MahjoubFlore Sicre de FontbruneSarah MaylinFrançois SimonCatherine ScieuxGérard SociéMarie-Christine MazeronJérôme LeGoff
作者单位: 1Université de Paris Diderot, INSERM U976, Paris, France
2Laboratoire de Microbiologie, Hôpital Saint-Louis, APHP, Paris, France
3Université de Tours, INSERM U1259, Tours, France
4Hematology and Transplantation Unit, Hôpital Saint-Louis, APHP, Paris, France
刊名: Virology Journal, 2020, Vol.17 (Suppl 1), pp.862-869
来源数据库: Springer Nature Journal
DOI: 10.1186/s12985-020-1285-7
关键词: EBVQuantitative PCRHematopoietic stem cell transplantMonitoringRituximab
英文摘要: Abstract(#br)Background(#br)Standardized and sensitive assays for Epstein Barr Virus (EBV) are needed to define universal cutoff for treatment initiation in allogeneic hematopoietic stem cells transplant recipients. In a context of accreditation and the availability of EBV international standard, we evaluated the Abbott RealTime EBV (RT) assay for EBV quantification in whole blood. Methods(#br)The RT assay was compared on 282 prospective clinical samples with the Artus EBV PCR Kit V1 assay (V1) and we analyzed the kinetics of EBV load in 11 patients receiving rituximab treatment. Results(#br)The estimated limit of detection was 88 IU/mL. The assay was linear (r2 = 0.9974) in the range of all samples tested (100 to 1,000,000 IU/mL). Intra-assay coefficients of variation (CV)...
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影响因子:2.092 (2012)

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