Porcine β-defensin 2 inhibits proliferation of pseudorabies virus in vitro and in transgenic mice
作者: Jing HuangYanhua QiAntian WangChao HuangXiao LiuXi YangLu LiRui Zhou
作者单位: 1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 430070, Wuhan, China
2Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, 430070, Wuhan, China
3Chongqing Academy of Animal Sciences, 402460, Chongqing, China
4Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, 430070, Wuhan, China
5International Research Center for Animal Disease, Ministry of Science and Technology of the People’s Republic of China, 430070, Wuhan, China
刊名: Virology Journal, 2020, Vol.17 (1), pp.369-391
来源数据库: Springer Nature Journal
DOI: 10.1186/s12985-020-1288-4
关键词: Porcine β-defensin 2Pseudorabies virusAntiviral abilityTransgenic mice
原始语种摘要: Abstract(#br)Background(#br)Porcine β-defensin 2 (PBD-2), produced by host cells, is an antimicrobial cysteine-rich cationic peptide with multi-functions. Previous studies have demonstrated that PBD-2 can kill various bacteria, regulate host immune responses and promote growth of piglets. However, the antiviral role of PBD-2 is rarely investigated. This study aimed to reveal the antiviral ability of PBD-2 against pseudorabies virus (PRV), the causative pathogen of Aujeszky’s disease, in PK-15 cells and in a PBD-2 expressing transgenic (TG) mouse model. Methods(#br)In this study, the cytotoxicity of PBD-2 on PK-15 cells was measured by CCK-8 assay. PK-15 cells were incubated with PRV pre-treated with different concentrations of PBD-2 and PRV titers in cell culture supernatants were...
全文获取路径: Springer Nature  (合作)
影响因子:2.092 (2012)