Improvement in RNA quantity and quality in cervico-vaginal cytology
作者: Gun Oh. ChongHyung Soo HanSeon Duk LeeYoon Hee Lee
作者单位: 1Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
2Department of Obstetrics and Gynecology, Kyungpook National University Chilgok Hospital, Daegu, Republic of Korea
3Molecular Diagnostics and Imaging Center, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
4Department of Physiology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
刊名: Virology Journal, 2020, Vol.17 (1), pp.108-115
来源数据库: Springer Nature Journal
DOI: 10.1186/s12985-020-1282-x
关键词: Cervico-vaginal cytologyMRNAQuantityQuality
英文摘要: Abstract(#br)The separation of exfoliated cells from the brushes used during cervico-vaginal smears is difficult, a problem which may affect the quality of ribonucleic acid (RNA) extracted. We compared the results of RNA extraction from cervico-vaginal cytology samples according to the type of tubes, preservative solutions, and storage temperature. The samples included exfoliated cervico-vaginal cytological specimens from patients with human papilloma virus 16, positive for cervical intraepithelial neoplasia or cervical cancer. Exfoliated cells were obtained by shaking a brush in a conventional rigid vial tube or squeezing the brush in a soft vial tube. RNA quantity and quality were compared between the two tubes. The concentration and purity of RNA (A260/A280 and A260/A230 ratios) was...
全文获取路径: Springer Nature  (合作)
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影响因子:2.092 (2012)

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