Alanyl-glutamine but not glycyl-glutamine improved the proliferation of enterocytes as glutamine substitution in vitro
作者: Bie TanHonglan LiuGuangzu HeHao XiaoDingfu XiaoYanhong LiuJianping WuJun FangYulong Yin
作者单位: 1Chinese Academy of Sciences
2Hunan Agricultural University
3University of California
刊名: Amino Acids, 2017, Vol.49 (12), pp.2023-2031
来源数据库: Springer Nature Journal
DOI: 10.1007/s00726-017-2460-z
关键词: GlutamineDipeptideMitochondrial bioenergeticsProtein turnoverEnterocyte
英文摘要: The synthetic dipeptides alanyl-glutamine (Ala-Gln) and glycyl-glutamine (Gly-Gln) are used as Gln substitution to provide energy source in the gastrointestinal tract due to their high solubility and stability. This study aimed to investigate the effects of Gln, Ala-Gln and Gly-Gln on mitochondrial respiration and protein turnover of enterocytes. Intestinal porcine epithelial cells (IPEC-J2) were cultured for 2 days in Dulbecco’s modified Eagle’s-F12 Ham medium (DMEM-F12) containing 2.5 mM Gln, Ala-Gln or Gly-Gln. Results from 5-ethynyl-2′-deoxyuridine incorporation and flow cytometry analysis indicated that there were no differences in proliferation between free Gln and Ala-Gln-treated cells, whereas Gly-Gln treatment inhibited the cell growth compared with Gln treatment. Significantly...
原始语种摘要: The synthetic dipeptides alanyl-glutamine (Ala-Gln) and glycyl-glutamine (Gly-Gln) are used as Gln substitution to provide energy source in the gastrointestinal tract due to their high solubility and stability. This study aimed to investigate the effects of Gln, Ala-Gln and Gly-Gln on mitochondrial respiration and protein turnover of enterocytes. Intestinal porcine epithelial cells (IPEC-J2) were cultured for 2 days in Dulbecco’s modified Eagle’s-F12 Ham medium (DMEM-F12) containing 2.5 mM Gln, Ala-Gln or Gly-Gln. Results from 5-ethynyl-2′-deoxyuridine incorporation and flow cytometry analysis indicated that there were no differences in proliferation between free Gln and Ala-Gln-treated cells, whereas Gly-Gln treatment inhibited the cell growth compared with Gln treatment. Significantly...
全文获取路径: Springer Nature  (合作)
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来源刊物:
影响因子:3.914 (2012)

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关键词翻译
关键词翻译
  • glutamine 谷氨酰胺
  • substitution 同位素置换
  • vitro 体外
  • protein 蛋白质
  • proliferation 增生
  • turnover 代谢回转
  • treatment 处理
  • cells 麻风细胞
  • respiration 呼吸
  • increased 增加