Antitumor Efficacy of SLPI Promoter-Controlled Expression of Artificial microRNA Targeting EGFR in a Squamous Cell Carcinoma Cell Line
作者: Jia ChenShoude ZhangYi LinBeibei YangJiang Cao
作者单位: 1Zhejiang University
刊名: Pathology & Oncology Research, 2017, Vol.23 (4), pp.829-835
来源数据库: Springer Nature Journal
DOI: 10.1007/s12253-016-0160-8
关键词: EGFRGene therapyMicroRNARecombinant adenovirusSLPI
英文摘要: The purpose of this study was to develop a recombinant adenovirus with secretory leukoprotease inhibitor (SLPI) promoter-controlled expression for gene therapy of squamous cell carcinoma (SCC). An artificial microRNA targeting epidermal growth factor receptor (EGFR) was designed, and used to construct a replication-defective recombinant adenovirus with SLPI promoter-controlled expression. The silencing efficiency of this vector (Ad-SLPI-EGFRamiR) was detected in Hep-2 cells. Western blotting showed that the expression of 170 kD EGFR was significantly reduced in Hep-2 cells 72 h after infection with Ad-SLPI-EGFRamiR. At a multiplicity of infection (MOI) of 200 pfu/cell, proliferation of Hep-2 cells was highly inhibited by Ad-SLPI-EGFRamiR (inhibition rate: ~70%). The apoptosis rate of...
原始语种摘要: The purpose of this study was to develop a recombinant adenovirus with secretory leukoprotease inhibitor (SLPI) promoter-controlled expression for gene therapy of squamous cell carcinoma (SCC). An artificial microRNA targeting epidermal growth factor receptor (EGFR) was designed, and used to construct a replication-defective recombinant adenovirus with SLPI promoter-controlled expression. The silencing efficiency of this vector (Ad-SLPI-EGFRamiR) was detected in Hep-2 cells. Western blotting showed that the expression of 170 kD EGFR was significantly reduced in Hep-2 cells 72 h after infection with Ad-SLPI-EGFRamiR. At a multiplicity of infection (MOI) of 200 pfu/cell, proliferation of Hep-2 cells was highly inhibited by Ad-SLPI-EGFRamiR (inhibition rate: ~70%). The apoptosis rate of...
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影响因子:1.555 (2012)

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关键词翻译
关键词翻译
  • adenovirus 腺病毒
  • leukoprotease 白细胞蛋白酶
  • targeting 导向目标
  • cells 麻风细胞
  • defective 有缺陷的
  • squamous 具鳞的
  • controlled 受控
  • promoter 催化剂
  • carcinoma 癌症
  • expression 表示