Detection of human neutrophil elastase by aptamer affinity capillary electrophoresis coupled with laser-induced fluorescence using specified site fluorescently labeled aptamer
作者: Yunlong BaiHailin WangQiang Zhao
作者单位: 1Shanxi University
2Chinese Academy of Sciences
3University of Chinese Academy of Sciences
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (29), pp.6843-6849
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0645-7
关键词: Human neutrophil elastaseAptamerCapillary electrophoresisLaser-induced fluorescenceAffinity bindingProtein detection
英文摘要: As a multifunctional serine protease, human neutrophil elastase (HNE) plays critical roles in a variety of physiopathological processes, such as acute lung injury, emphysema, atherosclerosis, and arthritis. The quantification of HNE is important in many applications. In this paper, we report an aptamer affinity capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) assay for detection of HNE using a tetramethylrhodamine (TMR)-labeled DNA aptamer probe. The affinity complex of HNE and DNA aptamer probe was well separated from the unbound aptamer probe in CE separation based on the difference of electrophoretic mobility. Broad complex peaks appeared due to possible multiple binding. The 45-mer aptamer having TMR labeling on the 40th T base was used as affinity probe, as...
原始语种摘要: As a multifunctional serine protease, human neutrophil elastase (HNE) plays critical roles in a variety of physiopathological processes, such as acute lung injury, emphysema, atherosclerosis, and arthritis. The quantification of HNE is important in many applications. In this paper, we report an aptamer affinity capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) assay for detection of HNE using a tetramethylrhodamine (TMR)-labeled DNA aptamer probe. The affinity complex of HNE and DNA aptamer probe was well separated from the unbound aptamer probe in CE separation based on the difference of electrophoretic mobility. Broad complex peaks appeared due to possible multiple binding. The 45-mer aptamer having TMR labeling on the 40th T base was used as affinity probe, as...
全文获取路径: Springer Nature  (合作)
分享到:
来源刊物:
影响因子:3.659 (2012)

×
关键词翻译
关键词翻译
  • affinity 亲和力
  • elastase 弹性蛋白酶
  • neutrophil 中性白细胞
  • labeled 示踪的
  • induced 感应的
  • capillary 毛状的
  • human 人的
  • fluorescence 荧光
  • coupled 耦合
  • complex 超群