Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12
作者: Laura E. SellarsJack A. BryantMaría-Antonia Sánchez-RomeroEugenio Sánchez-MoránStephen J. W. BusbyDavid J. Lee
作者单位: 1University of Birmingham
2Universidad de Sevilla
3Birmingham City University
刊名: BMC Microbiology, 2017, Vol.17 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12866-017-1079-2
关键词: FROSGFPFluorescent microscopyChromosomeNucleoidEscherichia coli
英文摘要: In bacteria, many transcription activator and repressor proteins regulate multiple transcription units that are often distally distributed on the bacterial genome. To investigate the subcellular location of DNA bound proteins in the folded bacterial nucleoid, fluorescent reporters have been developed which can be targeted to specific DNA operator sites. Such Fluorescent Reporter-Operator System (FROS) probes consist of a fluorescent protein fused to a DNA binding protein, which binds to an array of DNA operator sites located within the genome. Here we have developed a new FROS probe using the Escherichia coli MalI transcription factor, fused to mCherry fluorescent protein. We have used this in combination with a LacI repressor::GFP protein based FROS probe to assess the cellular location...
原始语种摘要: In bacteria, many transcription activator and repressor proteins regulate multiple transcription units that are often distally distributed on the bacterial genome. To investigate the subcellular location of DNA bound proteins in the folded bacterial nucleoid, fluorescent reporters have been developed which can be targeted to specific DNA operator sites. Such Fluorescent Reporter-Operator System (FROS) probes consist of a fluorescent protein fused to a DNA binding protein, which binds to an array of DNA operator sites located within the genome. Here we have developed a new FROS probe using the Escherichia coli MalI transcription factor, fused to mCherry fluorescent protein. We have used this in combination with a LacI repressor::GFP protein based FROS probe to assess the cellular location...
全文获取路径: Springer Nature  (合作)
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影响因子:3.104 (2012)

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关键词翻译
关键词翻译
  • fluorescent 萤光的
  • Escherichia 埃希氏菌属
  • reporter 报告生成程序
  • operator 话务员
  • repressor 阻遏因子
  • probes 探针
  • protein 蛋白质
  • nucleoid 类核
  • location 定位
  • fused 熔融的