Characteristics of induced mutations in offspring derived from irradiated mouse spermatogonia and mature oocytes
作者: Yasunari SatohJun-ichi AsakawaMayumi NishimuraTony KuoNorio ShinkaiHarry M. CullingsYohei MinakuchiJun SeseAtsushi ToyodaYoshiya ShimadaNori NakamuraArikuni Uchimura
作者单位: 1Department of Molecular Biosciences, Radiation Effects Research Foundation, 5-2 Hijiyama Park, 732-0815, Minami-ku, Hiroshima, Japan
2Department of Radiation Effects Research, National Institute of Radiological Sciences (NIRS), National Institutes for Quantum and Radiological Science and Technology (QST), 263-8555, Chiba, Japan
3Artificial Intelligence Research Center, AIST, 2-3-26 Aomi, 135-0064, Koto-ku, Tokyo, Japan
4Real World Big-Data Computation Open Innovation Laboratory, AIST-Tokyo Tech, 2-12-1 Okayama, 152-8550, Meguro-ku, Tokyo, Japan
5Department of Statistics, Radiation Effects Research Foundation, 5-2 Hijiyama Park, 732-0815, Minami-ku, Hiroshima, Japan
6Comparative Genomics Laboratory, National Institute of Genetics, 411-8540, Mishima, Japan
7Humanome Lab, Inc., L-HUB 3F, 1-4, Shumomiyabi-cho, 162-0822, Sinjuku-ku, Tokyo, Japan
8Department of Radiological Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 116-8551, Tokyo, Japan
9Executive Director, QST, 263-8555, C
刊名: Scientific Reports, 2020, Vol.10 (1), pp.84-87
来源数据库: Springer Nature Journal
DOI: 10.1038/s41598-019-56881-2
原始语种摘要: Abstract(#br)The exposure of germ cells to radiation introduces mutations in the genomes of offspring, and a previous whole-genome sequencing study indicated that the irradiation of mouse sperm induces insertions/deletions (indels) and multisite mutations (clustered single nucleotide variants and indels). However, the current knowledge on the mutation spectra is limited, and the effects of radiation exposure on germ cells at stages other than the sperm stage remain unknown. Here, we performed whole-genome sequencing experiments to investigate the exposure of spermatogonia and mature oocytes. We compared de novo mutations in a total of 24 F1 mice conceived before and after the irradiation of their parents. The results indicated that radiation exposure, 4 Gy of gamma rays, induced 9.6...
全文获取路径: Springer Nature  (合作)
影响因子:2.927 (2012)