Thermal-Induced Denaturation and Aggregation Behavior of Lysozyme and Bovine Serum Albumin: a Thermodynamic and Structural Study
作者: Aline Sanches PerezCristiano Luis Pinto Oliveira
作者单位: 1University of São Paulo
刊名: Brazilian Journal of Physics, 2017, Vol.47 (5), pp.524-531
来源数据库: Springer Nature Journal
DOI: 10.1007/s13538-017-0520-1
关键词: ProteinsSmall-angle X-ray scatteringSAXSModelingProtein structureDSC
英文摘要: Solution studies permit a direct investigation of the particles on a well-defined environment. Fluorescence, circular dichroism, scattering, and calorimetry provide, individually, very important information among the protein structure, overall shape, and thermodynamic equilibrium. In this work, a combination of these techniques is presented for the study of denaturation induced by temperature of two well-known proteins, Henn Egg lysozyme and bovine serum albumin. A detailed thermodynamic and structural investigation is shown for these proteins, providing interesting information on the thermal-induced changes in the protein structure and aggregation behavior.
原始语种摘要: Solution studies permit a direct investigation of the particles on a well-defined environment. Fluorescence, circular dichroism, scattering, and calorimetry provide, individually, very important information among the protein structure, overall shape, and thermodynamic equilibrium. In this work, a combination of these techniques is presented for the study of denaturation induced by temperature of two well-known proteins, Henn Egg lysozyme and bovine serum albumin. A detailed thermodynamic and structural investigation is shown for these proteins, providing interesting information on the thermal-induced changes in the protein structure and aggregation behavior.
全文获取路径: Springer Nature  (合作)
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来源刊物:
影响因子:0.598 (2012)

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关键词翻译
关键词翻译
  • proteins 蛋白质
  • denaturation 变性
  • Fluorescence 荧光
  • lysozyme 溶菌酶
  • investigation 研究
  • interesting 有趣的
  • information 报告
  • known 己知
  • these 这些的
  • thermodynamic 热力学的