Synthetic cell–cell communication in a three-species consortium for one-step vitamin C fermentation
作者: En-Xu WangYu LiuQian MaXiu-Tao DongMing-Zhu DingYing-Jin Yuan
作者单位: 1Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
2Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University
刊名: Biotechnology Letters, 2019, Vol.41 (8-9), pp.951-961
来源数据库: Springer Nature Journal
DOI: 10.1007/s10529-019-02705-2
关键词: One-step fermentationProgrammed cell deathQuorum-sensing2-keto- l -gulonic acid
原始语种摘要: Abstract(#br) Objectives(#br)A three-species consortium for one-step fermentation of 2-keto- l -gulonic acid (2-KGA) was constructed to better strengthen the cell–cell communication. And the programmed cell death module based on the LuxI/LuxR quorum-sensing (QS) system was established in Gluconobacter oxydans to reduce the competition that between G. oxydans and Ketogulonicigenium vulgare .(#br) Results(#br)By constructing and optimizing the core region of the promoter, which directly regulated the expression of lethal ccdB genes in QS system, IR3C achieved the best lethal effect. The consortium of IR3C- K. vulgare – Bacillus megaterium (abbreviated as 3C) achieved the highest 2-KGA titer (68.80 ± 4.18 g/l), and the molar conversion rate was 80.7% within 36 h in 5 l fermenter. Metabolomic...
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影响因子:1.853 (2012)

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