Cryo-EM structures of the mammalian endo-lysosomal TRPML1 channel elucidate the combined regulation mechanism
作者: Sensen ZhangNingning LiWenwen ZengNing GaoMaojun Yang
作者单位: 1Tsinghua University
2Peking University
刊名: Protein & Cell, 2017, Vol.8 (11), pp.834-847
来源数据库: Springer Nature Journal
DOI: 10.1007/s13238-017-0476-5
关键词: MTRPML1Mucolipidosis type IVStructual comparisonsCombined regulation mechanism
英文摘要: TRPML1 channel is a non-selective group-2 transient receptor potential (TRP) channel with Ca2+ permeability. Located mainly in late endosome and lysosome of all mammalian cell types, TRPML1 is indispensable in the processes of endocytosis, membrane trafficking, and lysosome biogenesis. Mutations of TRPML1 cause a severe lysosomal storage disorder called mucolipidosis type IV (MLIV). In the present study, we determined the cryo-electron microscopy (cryo-EM) structures of Mus musculus TRPML1 (mTRPML1) in lipid nanodiscs and Amphipols. Two distinct states of mTRPML1 in Amphipols are added to the closed state, on which could represent two different confirmations upon activation and regulation. The polycystin-mucolipin domain (PMD) may sense the luminal/extracellular stimuli and...
原始语种摘要: TRPML1 channel is a non-selective group-2 transient receptor potential (TRP) channel with Ca2+ permeability. Located mainly in late endosome and lysosome of all mammalian cell types, TRPML1 is indispensable in the processes of endocytosis, membrane trafficking, and lysosome biogenesis. Mutations of TRPML1 cause a severe lysosomal storage disorder called mucolipidosis type IV (MLIV). In the present study, we determined the cryo-electron microscopy (cryo-EM) structures of Mus musculus TRPML1 (mTRPML1) in lipid nanodiscs and Amphipols. Two distinct states of mTRPML1 in Amphipols are added to the closed state, on which could represent two different confirmations upon activation and regulation. The polycystin-mucolipin domain (PMD) may sense the luminal/extracellular stimuli and...
全文获取路径: Springer Nature  (合作)
分享到:
来源刊物:
影响因子:3.22 (2012)

×
关键词翻译
关键词翻译
  • mammalian 哺乳动物
  • endo 
  • combined 组合
  • regulation 
  • mucolipidosis 假胡尔勒氏多种营养不良
  • mechanism 机构
  • structural 构造
  • lysosome 溶酶体
  • sense 方向
  • channel 槽水道