Improving repeatability of capillary electrophoresis—a critical comparison of ten different capillary inner surfaces and three criteria of peak identification
作者: Paweł Mateusz NowakMichał WoźniakiewiczMarta GładyszMagdalena JanusPaweł Kościelniak
作者单位: 1Jagiellonian University in Kraków
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (18), pp.4383-4393
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0382-y
关键词: Capillary electrophoresisCapillary coatingElectrophoretic mobilityMicellar electrokinetic chromatographyRelative migration timesRepeatability
原始语种摘要: A poor repeatability of migration times caused by the fluctuations of electroosmotic flow (EOF) is an inherent weakness of capillary electrophoresis. Most researchers endeavor to prevent this problem using relative migration times or various capillary coatings which are expensive and not easy in comparison. Herein, we present an original approach to this problem: we apply a model sample designed to induce significant EOF instability, in order to critically compare ten capillary types with different physicochemical characteristics. Moreover, we accompany capillary modification with the evaluation of various criteria of peak identification: migration time, migration times ratio, and electrophoretic mobility. Our results show a great effectiveness of a dynamic coating in the stabilization of...
全文获取路径: Springer Nature  (合作)
影响因子:3.659 (2012)

  • capillary 毛状的
  • times 
  • migration 迁移
  • repeatability 可重复性
  • identification 辨认
  • relative 相对的
  • criteria 准则
  • mobility 活动性
  • electroosmotic 电渗的
  • capillaries 毛细管