Prediction of Escherichia coli expression performance in microtiter plates by analyzing only the temporal development of scattered light during culture
作者: Tobias LadnerMartina MühlmannAndreas SchulteGeorg WandreyJochen Büchs
作者单位: 1RWTH Aachen University
刊名: Journal of Biological Engineering, 2017, Vol.11 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s13036-017-0064-5
关键词: BioLectorEscherichia coliMicrotiter plateInduction optimizationScattered light
原始语种摘要: Escherichia coli is often used for recombinant protein production. The expression of recombinant proteins negatively affects the microbial growth, thus, a balance between protein expression and biomass formation is preferable to reach high product- and space-time-yield. Already in screening experiments, suboptimal conditions causing too weak or too strong induction must be avoided. High-throughput screening devices such as the BioLector are often applied for screening experiments. The BioLector allows optical online monitoring of each well in a continuously orbitally shaken microtiter plate via scattered light and fluorescence measurements. This technique enables a fast identification of promising clones. However, to determine the expression performance of non-fluorescent products...
全文获取路径: Springer Nature  (合作)

  • expression 表示
  • Escherichia 埃希氏菌属
  • scattered 散射的
  • light 
  • screening 筛分
  • fluorescent 萤光的
  • performance 性能
  • recombinant 重组体
  • protein 蛋白质
  • required 必需