Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy
作者: Rosemary A. BamfordAshley SmithJeremy MetzGeorgina GloverRichard W. TitballStefano Pagliara
作者单位: 1University of Exeter
刊名: BMC Biology, 2017, Vol.15 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12915-017-0465-4
关键词: Viable but non-culturable cellsMicrofluidicsEscherichia coliPersistersSingle-cellAntibiotic toleranceNon-growing cells
英文摘要: Clonal microbial populations often harbor rare phenotypic variants that are typically hidden within the majority of the remaining cells, but are crucial for the population’s resilience to external perturbations. Persister and viable but non-culturable (VBNC) cells are two important clonal bacterial subpopulations that can survive antibiotic treatment. Both persister and VBNC cells pose a serious threat to human health. However, unlike persister cells, which quickly resume growth following drug removal, VBNC cells can remain non-growing for prolonged periods of time, thus eluding detection via traditional microbiological assays. Therefore, understanding the molecular mechanisms underlying the formation of VBNC cells requires the characterization of the clonal population with single-cell...
原始语种摘要: Clonal microbial populations often harbor rare phenotypic variants that are typically hidden within the majority of the remaining cells, but are crucial for the population’s resilience to external perturbations. Persister and viable but non-culturable (VBNC) cells are two important clonal bacterial subpopulations that can survive antibiotic treatment. Both persister and VBNC cells pose a serious threat to human health. However, unlike persister cells, which quickly resume growth following drug removal, VBNC cells can remain non-growing for prolonged periods of time, thus eluding detection via traditional microbiological assays. Therefore, understanding the molecular mechanisms underlying the formation of VBNC cells requires the characterization of the clonal population with single-cell...
全文获取路径: Springer  (合作)
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影响因子:6.531 (2012)

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关键词翻译
关键词翻译
  • viable 有生活力的
  • remaining 剩余
  • fluorescent 萤光的
  • cells 麻风细胞
  • typically 典型地
  • bacterial 细菌的
  • microscopy 显微镜检查
  • Escherichia 埃希氏菌属
  • persister 冷持管
  • reporter 报告生成程序