Immobilization of Ulp1 protease on NHS-activated Sepharose: a useful tool for cleavage of the SUMO tag of recombinant proteins
作者: Qiujin LiangZhengzhi HuangYuan ZhangHongtao Li
作者单位: 1Southwest University
刊名: Biotechnology Letters, 2017, Vol.39 (7), pp.1025-1031
来源数据库: Springer Journal
DOI: 10.1007/s10529-017-2330-5
关键词: Enzymatic cleavageExtreme pHs toleranceImmobilizationThermal stabilityUbl-specific protease (Ulp1)
英文摘要: To fabricate an active and stable enzyme through covalent immobilization, a Ubl-specific protease (Ulp1) was used to cleave small ubiquitin-like modifier (SUMO) fusion proteins.
原始语种摘要: To fabricate an active and stable enzyme through covalent immobilization, a Ubl-specific protease (Ulp1) was used to cleave small ubiquitin-like modifier (SUMO) fusion proteins.
全文获取路径: Springer  (合作)
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影响因子:1.853 (2012)

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关键词翻译
关键词翻译
  • cleavage 解理
  • protease 朊酶
  • proteins 蛋白质
  • recombinant 重组体
  • specific 
  • small 小的
  • active 活动的
  • fabricate 伪造
  • cleave 劈开解理
  • stability 稳定性