Magnetite nanoparticles coated with chitosan and polyethylenimine as anion exchanger for sorptive enrichment of phosphopeptides
作者: Dilshad HussainSyed Ghulam MusharrafBatool FatimaAdeela SaeedFahmida JabeenMuhammad Naeem AshiqMuhammad Najam-ul-Haq
作者单位: 1Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University
2HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi
3Department of Biochemistry, Bahauddin Zakariya University
4Department of Chemistry, The Women University
5Institute of Chemical Sciences, Bahauddin Zakariya University
刊名: Microchimica Acta, 2019, Vol.186 (12), pp.1-10
来源数据库: Springer Nature Journal
DOI: 10.1007/s00604-019-3971-y
关键词: Affinity materialMagnetic iron oxide nanoparticlesAnion exchangerSelectivityNon-fat milkEgg-yolkHuman serumEndogenous phosphopeptides
英文摘要: Abstract(#br)An anion exchange solid-phase sorbent is described. Chitosan coated magnetite nanoparticles were modified with polyethylenimine which is positively charged at pH 3 and therefore can be used for the magnet-supported enrichment of phosphopeptides which are negatively charged at this pH value. A 2-step strategy was used to synthesize the sorbent. The materials were characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry and magnetic moment analysis. The anion exchanger was applied to extract phosphopeptides from a β-casein digest. Characteristic analytical figures include (a) a loading buffer of pH 3, (b) and elution buffer of pH 11, (c) a loading time of 5 min, (d) good...
全文获取路径: Springer Nature  (合作)
影响因子:3.434 (2012)