Quantitation of glutathione S -transferases in rice ( Oryza sativa L.) roots exposed to cadmium by liquid chromatography–tandem mass spectrometry using isotope-labeled wing peptides as an internal standard
作者: Zhenzhen CaoRenxiang MouZhaoyun CaoXiaoyan LinYouning MaZhiwei ZhuMingxue Chen
作者单位: 1China National Rice Research Institute
刊名: Plant Methods, 2017, Vol.13 (1)
来源数据库: Springer Journal
DOI: 10.1186/s13007-017-0214-2
关键词: Glutathione S -transferasesCadmiumLiquid chromatography–tandem mass spectrometrySignature peptideIsotope-labeled internal standard
英文摘要: Plant glutathione S -transferases (GSTs, EC 2.5.1.18) are multifunctional enzymes involved in heavy metal cellular detoxification by conjugating the tripeptide (g-Glu-Cys-Gly) glutathione to heavy metals. Previous studies demonstrated that individual rice GSTs were differentially induced by heavy metal exposure at the mRNA transcript level. However, little information is available concerning changes in protein concentration of rice GSTs under heavy metal stress. Because the correlation between changes in protein concentration and gene expression under abiotic stress is poor, direct determination of rice GSTs protein concentrations during cadmium (Cd) exposure is a more effective and reliable approach to explore possible mechanisms of rice Cd translocation and accumulation.
原始语种摘要: Plant glutathione S -transferases (GSTs, EC 2.5.1.18) are multifunctional enzymes involved in heavy metal cellular detoxification by conjugating the tripeptide (g-Glu-Cys-Gly) glutathione to heavy metals. Previous studies demonstrated that individual rice GSTs were differentially induced by heavy metal exposure at the mRNA transcript level. However, little information is available concerning changes in protein concentration of rice GSTs under heavy metal stress. Because the correlation between changes in protein concentration and gene expression under abiotic stress is poor, direct determination of rice GSTs protein concentrations during cadmium (Cd) exposure is a more effective and reliable approach to explore possible mechanisms of rice Cd translocation and accumulation.
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影响因子:2.667 (2012)

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关键词翻译
关键词翻译
  • sativa 漂白亚麻纤维卷
  • labeled 示踪的
  • roots 麻根
  • tandem 串联机
  • spectrometry 光谱测定
  • chromatography 色层分离法
  • internal 内部的
  • standard 标准
  • accumulation 堆积
  • translocation 移位