Sequence-based prediction of permissive stretches for internal protein tagging and knockdown
作者: Sabine OesterleTania Michelle RobertsLukas Andreas WidmerHarun MustafaSven PankeSonja Billerbeck
作者单位: 1ETH Zürich
2Swiss Institute of Bioinformatics
3Life Science Zürich Graduate School in Systems Biology
4Columbia University
刊名: BMC Biology, 2017, Vol.15 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12915-017-0440-0
关键词: Permissive siteInternal protein taggingTEV proteaseProtein knockdownsCell-free biotechnology
原始语种摘要: Internal tagging of proteins by inserting small functional peptides into surface accessible permissive sites has proven to be an indispensable tool for basic and applied science. Permissive sites are typically identified by transposon mutagenesis on a case-by-case basis, limiting scalability and their exploitation as a system-wide protein engineering tool.
全文获取路径: Springer Nature  (合作)
影响因子:6.531 (2012)

  • knockdown 击倒
  • tagging 锻尖
  • protein 蛋白质
  • permissive 许可的
  • transposon 转座子
  • typically 典型地
  • functional 功能的
  • mutagenesis 突变形成
  • biotechnology 生物工程
  • small 小的