Small phosphatidate phosphatase ( TtPAH2 ) of Tetrahymena complements respiratory function and not membrane biogenesis function of yeast PAH1
作者: Anoop Narayana PillaiSushmita ShuklaSudhanshu GautamAbdur Rahaman
作者单位: 1National Institute of Science Education and Research (NISER)
刊名: Journal of Biosciences, 2017, Vol.42 (4), pp.613-621
来源数据库: Springer Journal
DOI: 10.1007/s12038-017-9712-7
关键词: Lipid dropletLipinPAPPhosphatidate phosphataseTetrahymena
英文摘要: Phosphatidate phosphatases (PAH) play a central role in lipid metabolism and intracellular signaling. Herein, we report the presence of a low-molecular-weight PAH homolog in the single-celled ciliate Tetrahymena thermophila . In vitro phosphatase assay showed that TtPAH2 belongs to the magnesium-dependent phosphatidate phosphatase (PAP1) family. Loss of function of TtPAH2 did not affect the growth of Tetrahymena . Unlike other known PAH homologs, TtPAH2 did not regulate lipid droplet number and ER morphology. TtPAH2 did not rescue growth and ER/nuclear membrane defects of the pah1∆ yeast cells, suggesting that the phosphatidate phosphatase activity of the protein is not sufficient to perform these cellular functions. Surprisingly, TtPAH2 complemented the respiratory defect in the pah1∆...
原始语种摘要: Phosphatidate phosphatases (PAH) play a central role in lipid metabolism and intracellular signaling. Herein, we report the presence of a low-molecular-weight PAH homolog in the single-celled ciliate Tetrahymena thermophila . In vitro phosphatase assay showed that TtPAH2 belongs to the magnesium-dependent phosphatidate phosphatase (PAP1) family. Loss of function of TtPAH2 did not affect the growth of Tetrahymena . Unlike other known PAH homologs, TtPAH2 did not regulate lipid droplet number and ER morphology. TtPAH2 did not rescue growth and ER/nuclear membrane defects of the pah1∆ yeast cells, suggesting that the phosphatidate phosphatase activity of the protein is not sufficient to perform these cellular functions. Surprisingly, TtPAH2 complemented the respiratory defect in the pah1∆...
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来源刊物:
影响因子:1.759 (2012)

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关键词翻译
关键词翻译
  • phosphatidate 磷脂酸
  • yeast 酵母
  • phosphatase 磷酸酶
  • biogenesis 生源说
  • membrane 
  • respiration 呼吸
  • indicating 指示
  • droplet 液滴
  • function 函数
  • complements 互补商品