CYP1A1 based on metabolism of xenobiotics by cytochrome P450 regulates chicken male germ cell differentiation
作者: Dong LiMan WangShaoze ChengChen ZhangYilin WangWenhui ZhangRuifeng ZhaoChanghua SunYani ZhangBichun Li
作者单位: 1Yangzhou University
2Province Key Laboratory of Animal Breeding and Molecular Design
刊名: In Vitro Cellular & Developmental Biology - Animal, 2017, Vol.53 (4), pp.293-303
来源数据库: Springer Nature Journal
DOI: 10.1007/s11626-016-0108-z
关键词: RNA-SeqEmbryonic stem cellsPrimordial germ cellsSpermatogonial stem cellsMetabolism of xenobiotics by cytochrome P450Differentiation
原始语种摘要: This study aimed to explore the regulatory mechanism of metabolism of xenobiotics by cytochrome P450 during the differentiation process of chicken embryonic stem cells (ESCs) into spermatogonial stem cells (SSCs) and consummate the induction differentiation system of chicken embryonic stem cells (cESCs) into SSCs in vitro. We performed RNA-Seq in highly purified male ESCs, male primordial germ cells (PGCs), and SSCs that are associated with the male germ cell differentiation. Thereinto, the metabolism of xenobiotics by cytochrome P450 was selected and analyzed with Venny among male ESC vs male PGC, male PGC vs SSC, and male ESC vs SSC groups and several candidates differentially expressed genes (DEGs) were excavated. Finally, quantitative real-time PCR (qRT-PCR) detected related DEGs...
全文获取路径: Springer Nature  (合作)

  • cytochrome 细胞色素
  • xenobiotics 宾主共栖生物
  • metabolism 代谢
  • cells 麻风细胞
  • induced 感应的
  • primordial 原始的
  • process 过程
  • significantly 较大地
  • crucial 有决定性的