Dual effect of chloramphenicol peptides on ribosome inhibition
作者: Anthony BougasIoannis A. VlachogiannisDimitrios GatosStefan ArenzGeorge P. Dinos
作者单位: 1University of Patras
2Ludwig-Maximilians-University of Munich
刊名: Amino Acids, 2017, Vol.49 (5), pp.995-1004
来源数据库: Springer Journal
DOI: 10.1007/s00726-017-2406-5
关键词: Chloramphenicol-derivativesPeptidyl-tRNA analogsNascent peptidyl-tRNA mimicsRibosomal tunnelAntibiotics
英文摘要: Chloramphenicol peptides were recently established as useful tools for probing nascent polypeptide chain interaction with the ribosome, either biochemically, or structurally. Here, we present a new 10mer chloramphenicol peptide, which exerts a dual inhibition effect on the ribosome function affecting two distinct areas of the ribosome, namely the peptidyl transferase center and the polypeptide exit tunnel. According to our data, the chloramphenicol peptide bound on the chloramphenicol binding site inhibits the formation of both acetyl-phenylalanine-puromycin and acetyl-lysine-puromycin, showing, however, a decreased peptidyl transferase inhibition compared to chloramphenicol-mediated inhibition per se. Additionally, we found that the same compound is a strong inhibitor of green...
原始语种摘要: Chloramphenicol peptides were recently established as useful tools for probing nascent polypeptide chain interaction with the ribosome, either biochemically, or structurally. Here, we present a new 10mer chloramphenicol peptide, which exerts a dual inhibition effect on the ribosome function affecting two distinct areas of the ribosome, namely the peptidyl transferase center and the polypeptide exit tunnel. According to our data, the chloramphenicol peptide bound on the chloramphenicol binding site inhibits the formation of both acetyl-phenylalanine-puromycin and acetyl-lysine-puromycin, showing, however, a decreased peptidyl transferase inhibition compared to chloramphenicol-mediated inhibition per se. Additionally, we found that the same compound is a strong inhibitor of green...
全文获取路径: Springer  (合作)
分享到:
来源刊物:
影响因子:3.914 (2012)

×
关键词翻译
关键词翻译
  • ribosome 核糖体
  • effect 效应
  • peptidyl 肽酰
  • inhibition 抑制
  • inhibitory 禁止的
  • peptide 
  • puromycin 嘌呤霉素
  • blocking 阻塞
  • tunnel 坑道
  • transcription 地名转写