Expression and purification of an FGF9 fusion protein in E. coli , and the effects of the FGF9 subfamily on human hepatocellular carcinoma cell proliferation and migration
作者: Shen WangHaipeng LinTiantian ZhaoSisi HuangDavid G. FernigNuo XuFenfang WuMi ZhouChao JiangHaishan Tian
作者单位: 1Wenzhou Medical University
2University of Liverpool
3Wenzhou University
刊名: Applied Microbiology and Biotechnology, 2017, Vol.101 (21), pp.7823-7835
来源数据库: Springer Journal
DOI: 10.1007/s00253-017-8468-1
关键词: Recombinant human FGF9Fusion expressionTEV protease cleavageMitogen activityHuH7 cellsSignal transduction mechanism
英文摘要: Fibroblast growth factor (FGF) 9 has oncogenic activity and plays an important role in the development of ovarian, lung, prostate, and gastric cancers. In the present study, with the aim of reducing the cost of utilizing growth factors in cancer research, a simple and efficient method for the preparation of recombinant human (rh)FGF9 in Escherichia coli was established. The rhFGF9 fusion protein (6 × His-TEV-rhFGF9) and the native protein released by tobacco etch virus (TEV) protease were obtained using a Ni-NTA system, with > 95% purity. Both purified forms of rhFGF9, with and without fusion tags, significantly stimulated the proliferation of NIH3T3 cells. The FGF9 subfamily, including FGF9, FGF16, and FGF20, in addition to rhFGF16, rhFGF9, and rhFGF20, were shown to stimulate the...
原始语种摘要: Fibroblast growth factor (FGF) 9 has oncogenic activity and plays an important role in the development of ovarian, lung, prostate, and gastric cancers. In the present study, with the aim of reducing the cost of utilizing growth factors in cancer research, a simple and efficient method for the preparation of recombinant human (rh)FGF9 in Escherichia coli was established. The rhFGF9 fusion protein (6 × His-TEV-rhFGF9) and the native protein released by tobacco etch virus (TEV) protease were obtained using a Ni-NTA system, with > 95% purity. Both purified forms of rhFGF9, with and without fusion tags, significantly stimulated the proliferation of NIH3T3 cells. The FGF9 subfamily, including FGF9, FGF16, and FGF20, in addition to rhFGF16, rhFGF9, and rhFGF20, were shown to stimulate the...
全文获取路径: Springer  (合作)
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来源刊物:
影响因子:3.689 (2012)

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关键词翻译
关键词翻译
  • hepatocellular 肝细胞性的
  • coli 大肠菌
  • protein 蛋白质
  • carcinoma 癌症
  • proliferation 增生
  • migration 迁移
  • subfamily 亚科
  • human 人的
  • FGF 成纤维细胞生长因子
  • transduction 转导